other:
Article Title: RNF186 controls glucose metabolism in metabolic dysfunction-associated fatty liver disease
Article Snippet: Primary antibodies specific for the following proteins were purchased from Cell Signaling Technology: AKT (#4691), p-AKT (Ser473) (#4060), p-GSK3β (#9322), GSK3β (#12456), Fasn (#3180), GCK (#3782), SCD1 (#2794), IRS-1 (#2382), p-IRS-1 (#2388), PI3K (#4292), p-PI3K (#4288), FoxO1 (#2880), p-FoxO1 (#9461), JNK (#9252), p-JNK (#4668), GS (#3886), Ubiqutin (#3936), and HKII (#2867), p-eIF2α (#3398), eIF2α (#9722).
Article Title: RNF186 controls glucose metabolism in metabolic dysfunction-associated fatty liver disease.
Article Snippet: Primary antibodies specific for the following proteins were purchased from Cell Signaling Technology: AKT (#4691), p-AKT (Ser473) (#4060), p-GSK3β (#9322), GSK3β (#12456), Fasn (#3180), GCK (#3782), SCD1 (#2794), IRS-1 (#2382), p-IRS-1 (#2388), PI3K (#4292), p-PI3K (#4288), FoxO1 (#2880), p-FoxO1 (#9461), JNK (#9252), p-JNK (#4668), GS (#3886), Ubiqutin (#3936), and HKII (#2867), p-eIF2α (#3398), eIF2α (#9722).
Incubation:Article Title: Revealing the Biochemical Regulations of L-PGDS in Hepatic Insulin-Resistance using HepG2 cells.
Article Snippet: Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common chronic liver disease, and its prevalence poses a serious health threat globally.. MASLD is a multifactorial hepatic disorder, but insulin resistance is a key player.. Our prior in vivo studies revealed that the absence of lipocalin prostaglandin D2 synthase (L-PGDS) leads to the development of MASLD, often coexisting with insulin resistance.
Article Title: Gut glucose assimilation governs glucose homeostasis through reciprocal interaction between glucose and amino acid transporter.
Article Snippet: Equal amounts of protein (20 μg per lane) were separated by SDS–PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, Millipore). .. After blocking, the membranes were incubated with the following primary antibodies: p-IRS-1 (Ser307) (2381s, Cell Signaling Technology), p-IRS-1 (Tyr1222) (3066s, Cell Signaling Technology), IRS-1 (2382s, Cell Signaling Technology), p-PI3K (17366s, Cell Signaling Technology), PI3K (ab278545, Abcam), p-Akt (4060s, Cell Signaling Technology), Akt (4691s, Cell Signaling Technology), β-actin (60009-1-Ig, Proteintech), GAPDH (60004-1-Ig, Proteintech), GLUT1 (ab115730, Abcam), GLUT2 (PA5-77459, Invitrogen), GLUT3 (ab191071, Abcam), GLUT4 (ab188317, Abcam), GLUT5 (PA5-80024, Invitrogen), SGLT1 (PA5-37937, Invitrogen) and SLC6A19 (ab180516, Abcam). .. After washing and incubation with the appropriate horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology), the immune complexes were visualized using a chemiluminescence reagent.
Western Blot:Article Title: Revealing the Biochemical Regulations of L-PGDS in Hepatic Insulin-Resistance using HepG2 cells.
Article Snippet: Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common chronic liver disease, and its prevalence poses a serious health threat globally.. MASLD is a multifactorial hepatic disorder, but insulin resistance is a key player.. Our prior in vivo studies revealed that the absence of lipocalin prostaglandin D2 synthase (L-PGDS) leads to the development of MASLD, often coexisting with insulin resistance.
Article Title: Cytosolic phospholipase A2 links tau pathology to insulin signaling impairment in Alzheimer’s disease
Article Snippet: .. Tau-441 from rPeptide (Bogart, GA), and primary antibodies and reagents were purchased as follows: Anti-tau (T22) from Millipore Sigma (St. Louis, MO); antibodies against IR-α, IR-β, IRS-1, p-IRS-1 (Ser307), p-IRS-1 (Tyr895), cPLA2, p-cPLA2 and Cav-1 from Cell Signaling (Beverly, MA); dimethyl sulfoxide (DMSO), cOmplete protease inhibitor cocktail, PhosSTOP phosphatase inhibitor cocktail, β-mercaptoethanol (β-ME), Lipofectamine 3000 transfection reagent and cPLA2 siRNA from Thermo Fisher Scientific (Waltham, MA); Ham’s F-12 Nutrient Mix from Crystalgen Inc., (Commack, NY); EGM-2 MV BulletKit from Lonza (Walkersville, MD); penicillin/streptomycin (P/S), Hanks’ Balanced Salt Solution (HBSS), and Dulbecco’s phosphate-buffered saline (DPBS) from Life Technologies (Grand Island, NY); fibronectin, collagen from calf skin, laminin, and Minimum Essential Media Eagle (MEM) from Millipore Sigma (St. Louis, MO); papain and DNase from Worthington Biochemical (Lakewood, NJ); radio-immunoprecipitation assay (RIPA) buffer, BCA protein assay kit, SuperSignalTM West Femto maximum sensitivity substrate, SuperSignalTM West Pico Plus chemiluminescent substrate, and RestoreTM PLUS western-blot stripping buffer from Thermo Fisher Scientific (Waltham, MA); and Laemmli sample buffer and tris-buffered saline (TBS) from Bio-Rad (Hercules, CA). ..
Imaging:Article Title: Revealing the Biochemical Regulations of L-PGDS in Hepatic Insulin-Resistance using HepG2 cells.
Article Snippet: Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common chronic liver disease, and its prevalence poses a serious health threat globally.. MASLD is a multifactorial hepatic disorder, but insulin resistance is a key player.. Our prior in vivo studies revealed that the absence of lipocalin prostaglandin D2 synthase (L-PGDS) leads to the development of MASLD, often coexisting with insulin resistance.
Protease Inhibitor:Article Title: Cytosolic phospholipase A2 links tau pathology to insulin signaling impairment in Alzheimer’s disease
Article Snippet: .. Tau-441 from rPeptide (Bogart, GA), and primary antibodies and reagents were purchased as follows: Anti-tau (T22) from Millipore Sigma (St. Louis, MO); antibodies against IR-α, IR-β, IRS-1, p-IRS-1 (Ser307), p-IRS-1 (Tyr895), cPLA2, p-cPLA2 and Cav-1 from Cell Signaling (Beverly, MA); dimethyl sulfoxide (DMSO), cOmplete protease inhibitor cocktail, PhosSTOP phosphatase inhibitor cocktail, β-mercaptoethanol (β-ME), Lipofectamine 3000 transfection reagent and cPLA2 siRNA from Thermo Fisher Scientific (Waltham, MA); Ham’s F-12 Nutrient Mix from Crystalgen Inc., (Commack, NY); EGM-2 MV BulletKit from Lonza (Walkersville, MD); penicillin/streptomycin (P/S), Hanks’ Balanced Salt Solution (HBSS), and Dulbecco’s phosphate-buffered saline (DPBS) from Life Technologies (Grand Island, NY); fibronectin, collagen from calf skin, laminin, and Minimum Essential Media Eagle (MEM) from Millipore Sigma (St. Louis, MO); papain and DNase from Worthington Biochemical (Lakewood, NJ); radio-immunoprecipitation assay (RIPA) buffer, BCA protein assay kit, SuperSignalTM West Femto maximum sensitivity substrate, SuperSignalTM West Pico Plus chemiluminescent substrate, and RestoreTM PLUS western-blot stripping buffer from Thermo Fisher Scientific (Waltham, MA); and Laemmli sample buffer and tris-buffered saline (TBS) from Bio-Rad (Hercules, CA). ..
Transfection:Article Title: Cytosolic phospholipase A2 links tau pathology to insulin signaling impairment in Alzheimer’s disease
Article Snippet: .. Tau-441 from rPeptide (Bogart, GA), and primary antibodies and reagents were purchased as follows: Anti-tau (T22) from Millipore Sigma (St. Louis, MO); antibodies against IR-α, IR-β, IRS-1, p-IRS-1 (Ser307), p-IRS-1 (Tyr895), cPLA2, p-cPLA2 and Cav-1 from Cell Signaling (Beverly, MA); dimethyl sulfoxide (DMSO), cOmplete protease inhibitor cocktail, PhosSTOP phosphatase inhibitor cocktail, β-mercaptoethanol (β-ME), Lipofectamine 3000 transfection reagent and cPLA2 siRNA from Thermo Fisher Scientific (Waltham, MA); Ham’s F-12 Nutrient Mix from Crystalgen Inc., (Commack, NY); EGM-2 MV BulletKit from Lonza (Walkersville, MD); penicillin/streptomycin (P/S), Hanks’ Balanced Salt Solution (HBSS), and Dulbecco’s phosphate-buffered saline (DPBS) from Life Technologies (Grand Island, NY); fibronectin, collagen from calf skin, laminin, and Minimum Essential Media Eagle (MEM) from Millipore Sigma (St. Louis, MO); papain and DNase from Worthington Biochemical (Lakewood, NJ); radio-immunoprecipitation assay (RIPA) buffer, BCA protein assay kit, SuperSignalTM West Femto maximum sensitivity substrate, SuperSignalTM West Pico Plus chemiluminescent substrate, and RestoreTM PLUS western-blot stripping buffer from Thermo Fisher Scientific (Waltham, MA); and Laemmli sample buffer and tris-buffered saline (TBS) from Bio-Rad (Hercules, CA). ..
Saline:Article Title: Cytosolic phospholipase A2 links tau pathology to insulin signaling impairment in Alzheimer’s disease
Article Snippet: .. Tau-441 from rPeptide (Bogart, GA), and primary antibodies and reagents were purchased as follows: Anti-tau (T22) from Millipore Sigma (St. Louis, MO); antibodies against IR-α, IR-β, IRS-1, p-IRS-1 (Ser307), p-IRS-1 (Tyr895), cPLA2, p-cPLA2 and Cav-1 from Cell Signaling (Beverly, MA); dimethyl sulfoxide (DMSO), cOmplete protease inhibitor cocktail, PhosSTOP phosphatase inhibitor cocktail, β-mercaptoethanol (β-ME), Lipofectamine 3000 transfection reagent and cPLA2 siRNA from Thermo Fisher Scientific (Waltham, MA); Ham’s F-12 Nutrient Mix from Crystalgen Inc., (Commack, NY); EGM-2 MV BulletKit from Lonza (Walkersville, MD); penicillin/streptomycin (P/S), Hanks’ Balanced Salt Solution (HBSS), and Dulbecco’s phosphate-buffered saline (DPBS) from Life Technologies (Grand Island, NY); fibronectin, collagen from calf skin, laminin, and Minimum Essential Media Eagle (MEM) from Millipore Sigma (St. Louis, MO); papain and DNase from Worthington Biochemical (Lakewood, NJ); radio-immunoprecipitation assay (RIPA) buffer, BCA protein assay kit, SuperSignalTM West Femto maximum sensitivity substrate, SuperSignalTM West Pico Plus chemiluminescent substrate, and RestoreTM PLUS western-blot stripping buffer from Thermo Fisher Scientific (Waltham, MA); and Laemmli sample buffer and tris-buffered saline (TBS) from Bio-Rad (Hercules, CA). ..
Radio Immunoprecipitation:Article Title: Cytosolic phospholipase A2 links tau pathology to insulin signaling impairment in Alzheimer’s disease
Article Snippet: .. Tau-441 from rPeptide (Bogart, GA), and primary antibodies and reagents were purchased as follows: Anti-tau (T22) from Millipore Sigma (St. Louis, MO); antibodies against IR-α, IR-β, IRS-1, p-IRS-1 (Ser307), p-IRS-1 (Tyr895), cPLA2, p-cPLA2 and Cav-1 from Cell Signaling (Beverly, MA); dimethyl sulfoxide (DMSO), cOmplete protease inhibitor cocktail, PhosSTOP phosphatase inhibitor cocktail, β-mercaptoethanol (β-ME), Lipofectamine 3000 transfection reagent and cPLA2 siRNA from Thermo Fisher Scientific (Waltham, MA); Ham’s F-12 Nutrient Mix from Crystalgen Inc., (Commack, NY); EGM-2 MV BulletKit from Lonza (Walkersville, MD); penicillin/streptomycin (P/S), Hanks’ Balanced Salt Solution (HBSS), and Dulbecco’s phosphate-buffered saline (DPBS) from Life Technologies (Grand Island, NY); fibronectin, collagen from calf skin, laminin, and Minimum Essential Media Eagle (MEM) from Millipore Sigma (St. Louis, MO); papain and DNase from Worthington Biochemical (Lakewood, NJ); radio-immunoprecipitation assay (RIPA) buffer, BCA protein assay kit, SuperSignalTM West Femto maximum sensitivity substrate, SuperSignalTM West Pico Plus chemiluminescent substrate, and RestoreTM PLUS western-blot stripping buffer from Thermo Fisher Scientific (Waltham, MA); and Laemmli sample buffer and tris-buffered saline (TBS) from Bio-Rad (Hercules, CA). ..
Bicinchoninic Acid Protein Assay:Article Title: Cytosolic phospholipase A2 links tau pathology to insulin signaling impairment in Alzheimer’s disease
Article Snippet: .. Tau-441 from rPeptide (Bogart, GA), and primary antibodies and reagents were purchased as follows: Anti-tau (T22) from Millipore Sigma (St. Louis, MO); antibodies against IR-α, IR-β, IRS-1, p-IRS-1 (Ser307), p-IRS-1 (Tyr895), cPLA2, p-cPLA2 and Cav-1 from Cell Signaling (Beverly, MA); dimethyl sulfoxide (DMSO), cOmplete protease inhibitor cocktail, PhosSTOP phosphatase inhibitor cocktail, β-mercaptoethanol (β-ME), Lipofectamine 3000 transfection reagent and cPLA2 siRNA from Thermo Fisher Scientific (Waltham, MA); Ham’s F-12 Nutrient Mix from Crystalgen Inc., (Commack, NY); EGM-2 MV BulletKit from Lonza (Walkersville, MD); penicillin/streptomycin (P/S), Hanks’ Balanced Salt Solution (HBSS), and Dulbecco’s phosphate-buffered saline (DPBS) from Life Technologies (Grand Island, NY); fibronectin, collagen from calf skin, laminin, and Minimum Essential Media Eagle (MEM) from Millipore Sigma (St. Louis, MO); papain and DNase from Worthington Biochemical (Lakewood, NJ); radio-immunoprecipitation assay (RIPA) buffer, BCA protein assay kit, SuperSignalTM West Femto maximum sensitivity substrate, SuperSignalTM West Pico Plus chemiluminescent substrate, and RestoreTM PLUS western-blot stripping buffer from Thermo Fisher Scientific (Waltham, MA); and Laemmli sample buffer and tris-buffered saline (TBS) from Bio-Rad (Hercules, CA). ..
Stripping Membranes:Article Title: Cytosolic phospholipase A2 links tau pathology to insulin signaling impairment in Alzheimer’s disease
Article Snippet: .. Tau-441 from rPeptide (Bogart, GA), and primary antibodies and reagents were purchased as follows: Anti-tau (T22) from Millipore Sigma (St. Louis, MO); antibodies against IR-α, IR-β, IRS-1, p-IRS-1 (Ser307), p-IRS-1 (Tyr895), cPLA2, p-cPLA2 and Cav-1 from Cell Signaling (Beverly, MA); dimethyl sulfoxide (DMSO), cOmplete protease inhibitor cocktail, PhosSTOP phosphatase inhibitor cocktail, β-mercaptoethanol (β-ME), Lipofectamine 3000 transfection reagent and cPLA2 siRNA from Thermo Fisher Scientific (Waltham, MA); Ham’s F-12 Nutrient Mix from Crystalgen Inc., (Commack, NY); EGM-2 MV BulletKit from Lonza (Walkersville, MD); penicillin/streptomycin (P/S), Hanks’ Balanced Salt Solution (HBSS), and Dulbecco’s phosphate-buffered saline (DPBS) from Life Technologies (Grand Island, NY); fibronectin, collagen from calf skin, laminin, and Minimum Essential Media Eagle (MEM) from Millipore Sigma (St. Louis, MO); papain and DNase from Worthington Biochemical (Lakewood, NJ); radio-immunoprecipitation assay (RIPA) buffer, BCA protein assay kit, SuperSignalTM West Femto maximum sensitivity substrate, SuperSignalTM West Pico Plus chemiluminescent substrate, and RestoreTM PLUS western-blot stripping buffer from Thermo Fisher Scientific (Waltham, MA); and Laemmli sample buffer and tris-buffered saline (TBS) from Bio-Rad (Hercules, CA). ..
Blocking Assay:Article Title: Gut glucose assimilation governs glucose homeostasis through reciprocal interaction between glucose and amino acid transporter.
Article Snippet: Equal amounts of protein (20 μg per lane) were separated by SDS–PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, Millipore). .. After blocking, the membranes were incubated with the following primary antibodies: p-IRS-1 (Ser307) (2381s, Cell Signaling Technology), p-IRS-1 (Tyr1222) (3066s, Cell Signaling Technology), IRS-1 (2382s, Cell Signaling Technology), p-PI3K (17366s, Cell Signaling Technology), PI3K (ab278545, Abcam), p-Akt (4060s, Cell Signaling Technology), Akt (4691s, Cell Signaling Technology), β-actin (60009-1-Ig, Proteintech), GAPDH (60004-1-Ig, Proteintech), GLUT1 (ab115730, Abcam), GLUT2 (PA5-77459, Invitrogen), GLUT3 (ab191071, Abcam), GLUT4 (ab188317, Abcam), GLUT5 (PA5-80024, Invitrogen), SGLT1 (PA5-37937, Invitrogen) and SLC6A19 (ab180516, Abcam). .. After washing and incubation with the appropriate horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology), the immune complexes were visualized using a chemiluminescence reagent.
Expressing:Article Title: Effects of Intraperitoneal Colchicine on the Metabolic Consequences of High Fat Diet-Induced Obesity in Mice.
Article Snippet: Section on Growth and Obesity, Division of Intramural Research (DIR), Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH), Bethesda, MD 20892, USA; Section on Integrative Biophysics, DIR, NICHD, NIH, Bethesda, MD20892, USA; Current Address: Johns Hopkins Community Physicians at Howard County Medical Center, Johns Hopkins Medicine, Columbia, MD, USA
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